primary polyclonal rabbit antiinos antibody  (Novus Biologicals)

Journal: Italian Journal of Animal Science

Article Title: Protective effect of chitosan oligosaccharide against oxidative damage of peripheral blood mononuclear cells in dairy cows induced by diethylenetriamine/nitric oxide via NF-κB signalling pathway

doi: 10.1080/1828051x.2020.1772131

Figure Lengend Snippet: Figure 2. Effects of chitosan oligosaccharideon on the phosphorylation level of NF-kB pathway, the protein expression level of nitric oxide synthase (iNOS) and interleukin-1b (IL-1b). Expressions of IL-1b(A); phosphorylated IjB kinase b (P-IKKb) (B); phosphorylated inhibitor of nuclear factor kappa-Ba (P-IjBa) (C); phosphorylated nuclear factor kappa-Bp65(P-NF-jBp65) (D); iNOS (E), protein levels were detected by western blotting and normalised to beta-actin (b-Actin) levels. CTR¼ control treatment, without chitosan oligosacchar- ides addition; COS40, COS80, COS160, and COS320¼ treated with 40, 80, 160, and 320lg/mL chitosan oligosaccharides respectively.

Article Snippet: The membranes were incubated in the following primary antibodies: primary polyclonal rabbit anti-iNOS antibody (NBP1-97471, Novus Biological, USA), rabbit anti-IL-1b (Cell Signalling Technology, Boston, MA), anti-IKKb, monoclonal rat anti-IjBa, monoclonal rabbit anti-NF-jBp65 antibody(NF-jB pathway Sampler Kit 9963,CST, MA), anti-phospho-IKKb, anti-phospho-IjBa, and anti-phospho-NF-jBp65 (NF-jB pathway Sampler Kit 9963, CST, MA).

Techniques: Phospho-proteomics, Expressing, Western Blot, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β -Amyloid-Induced Rats Model of Alzheimer's Disease

doi: 10.1155/2020/5126457

Figure Lengend Snippet: Intensity relative of iNOS expression in brain homogenates among different rat groups. Sham-operated control (dPBS), β -amyloid control (dA β ), juice mixture + PBS (JPBS), juice mixture + β -amyloid (JA β ), and ibuprofen + β -amyloid (IBFA β ). ## Significant differences ( p < 0.01) as compared to the dA β group. Data are presented as mean ± standard error with n = 3 per treatment group.

Article Snippet: Then, the membrane was incubated with rabbit polyclonal anti-iNOS primary antibody (Abcam, USA; 1 : 1000 dilution) or rabbit monoclonal anti- β actin primary antibody (Abcam, USA; 1 : 1000 dilution) for 16 hours at 4°C and followed by 2 hours of incubation with HRP-conjugated anti-rabbit secondary antibody (Abcam, USA; 1 : 1000 dilution) at room temperature.

Techniques: Expressing, Control